High pressure tubing Use the amount that will be needed, including in-between-run time, plus a 20 to 30% allowance for time issues. No dummy modules need to be removed. This prompted me to issue a request (2) to you, the everyday users of LC, for information that might help clarify this situation. The pressure or leak test in Lab Advisor suggests the inlet valve may require replacement. Too many commands are pending in the fraction collector. Acetonitrile-resistant valves are now available for all Check Valve Cartridges that we currently offer - including Waters, Agilent and other brands. Location and illustration of the pH monitor The stirrer should move when the mixer is activated. Acetonitrile is used in analytical chemistry as a solvent or mobile phase. Incident: Blocked outlet check valve Two or several modules have the same Node ID. It is possible to install up to two I/O-box E9 when using KTA pure. The configuration is defined by the module's Node ID. Regenerate the pH electrode. - the tubing between the outlet valve and the fraction collector (2) J.W. The following procedure allows the cleaning of residue from the pump's check valves, often caused by polymerization of aged acetonitrile or salt-based solutions that may prevent the inlet and outlet valves from working correctly. See Section 7.8.4 Replace the UV monitor U9-M flow cell, on page 370 in system handbook. - Room temperature and limited overpressure is assumed. To prevent photochemical reactions and oxidations, use brown or amber bottles for CAN, and stick to minimal amounts. If you cannot resolvethe issue, contact your local Agilent Support representative for more help. Too high flow rate during usage of Drop sync, One or more Cassettes have empty positions. The cable between the pump and the ICU is not connected, Air trapped in the pump heads . CAS 75-05-08, molar mass 41.05 g/mol, and chemical formula CHCN. Verify that the UV cell is correctly installed. Clean the Drop sync diode windows and the nozzle. Required when: Empty positions must have connected jumpers. The tubing used should match the application needs. Navigate to Fraction collector:Fraction collector lamp and select Lamps On. Also, the problem is associated with acetonitrile in only one case was another solvent (tetrahydrofuran) mentioned as the organic present with sticking check valves. Remove the valve and make sure that the cable is connected. Figure 1 and Figure 2 show the hydraulic paths of the pumps. See Section 4.5 Install Loop valve V9-L, on page 148 in the system handbook. When the quality of the lot/batch is in doubt, change the lot/batch or the manufacturer/provider. In addition, wear and tear on systems under constant use by multiple end users is minimized, giving the system a longer life and better value for money. Manually turn Injection valve to Manual load position. This Information Applies To: Agilent G7120A Infinity II High Speed pump modules 4 In the displayed dialog in UNICORN, select the option Restart the system only and click OK. 1 In the displayed dialog in UNICORN, select the option Restart the system only and click OK. L9-1.2 has a 1.2 mm inner diameter and is designed for o.d. It has in general very good pressure limits, especially for water based buffers. See troubleshooting of pumps in Section 9.5 in the KTA pure System Handbook. The next question, of course, is if this change in surface is due to wear or to the deposit of something on the surface. These valves open to allow flow in one direction and close when flow stops or reverses. All rights reserved. Depending on pressure, tubing between pump head and pressure monitor needs to be changed. Check optical pathway and restart the instrument. The cable between the Injection valve and the ICU is not connected. Resolution: Check the flow path for air. Tricorn 10, High Performance Column Make sure that the Node ID is (00) for the primary box, and (01) for a potential secondary box. Incident: Leaking outlet check valve. If this error is recurrent, set the Fraction collector configuration manually in UNICORN. Open-end wrench 1/4 - 5/16 inch (p/n 87100510 ) Make sure that you have the MSDS available from your chemical distributor and/or databases on the internet. Use the recommended mixer size for the used flow rate. Also, buffers and salts that are solids dissolved in the mobile phase can form crystalline deposits inside the LC system if the liquid portion of the mobile phase is allowed to evaporate. Two different path lengths are available: 2 mm (default) and 5 mm, Reference capillary 1 and Tubing Kit 0.25, Syringe connector for pH valve and Injection valve, FromInlet valve A or Inlet valve AB to System pump A, FromInlet valve B or Inlet valve AB to System pump B. The cable between the Inlet valve and the ICU is not connected. See Section 7.8.4 Replace the UV monitor U9-M flow cell, on page 370 in system handbook. One of the troubles with trying to get a better handle on this problem is that data are very diffuse and tend to be confounded by changes that make it difficult to pin down a single reason why check-valve problems are so common. UV monitor U9-M together with UV monitor U9-L and or U9-T, 2nd, UV monitor U9-L together with UV monitor U9-L, 2nd. Replace UV flow cell. This article shows different options to troubleshoot low pressure or no flow issues in 1100/1200 series Isocratic/Quaternary/Binary pumps. See Conductivity monitor, on page 405 in the system handbook. To change the Mixer, see Section 7.8.2 Replace the Mixer, on page 366 in the system handbook. The module required for the instruction has not been enabled in the component selection in the administration module. Perform a System CIP. Over a year's time, I received inputs from 14 readers, scattered throughout the world in several different industries. If warning reappears, contact Service. The tubing is not mounted on delivery. Make sure that the automatic scanning is turned on in UNICORN software. Check the motor operation of the Mixer. Reference capillaries The cable between the Column valve and the ICU is not connected. Reducing valve, 0.2 bar with 2 tube connections 6 x 8 mm and integrated excess pressure safety device 0.5 bar: So if you do attempt to rebuild check valves, take extra care to work under the cleanest conditions possible. Two similar modules have been added to the instrument, for example two Inlet valve A. When sequences using ACN in the mobile phase are run overnight and in the morning no pressure or no flow is found, one of the causes is ACN polymerization. See Section 7.6.2 Perform System CIP, on page 325 in system handbook. The cable between the Mixer valve and the ICU is not connected. If this error is recurrent, generate a System error report and contact Service. The sealing surface is quite small: for example, in one patent (3), the sealing surface is 0.03 mm wide for a 1.5-mm diameter ball. 2 Change the Digital Out 1 signal between 1 and 0. The following steps will guide you to find out which part is causing How to Sonicate the Inlet and Outlet Valves of Agilent 1100, 1200 and 1260 Series Pump Heads. With the pressure on producing sample or results, the condition of your KTAdesign or Ettan system is critical and regular servicing will mean you can depend on your system to perform as expected. Check all connections for leaks. See Section 7.5.1 Clean the UV flow cell, on page 318 in system handbook. Clean the Cassette code reader diode window. An email will be sent to the address below containing a link to verify your email address. One of the headaches when using acetonitrile is the tendency for the ball and seat to get stuck actually bonded in the closed position. For cleaning of columns with HCl concentrations exceeding 0.1 M, manually fill a loop with HCl and inject the cleaning agent. Make sure that both the instrument and the buffers have reached the ambient temperature. If necessary, replace tubing. Use the Flow restrictor. Check the mixer chamber size and change the chamber if necessary. The illustration below shows the location of UV monitor U9-M with a detailed view of the monitor unit and detector. Check that the GE-logotype is facing outwards when the tray is inserted into the fraction collector. This error can only occur when the temperature compensation is turned on. 0.50 mm, Purge valve: Used to remove air from the pump, Connections to pump piston rinsing system: Tubing is connected between the pumps and the Pump piston rinsing system tube (6), Pump head: Encapsulates the inner parts of the pump, Tube Rack 40 30 mm, bowl, tube support, holder and guide, Tube Rack 175 12 mm, bowl, tube support, holder and guide, UniNet-9 D-type connector (for communication and power supply), Accumulator (back part of Dispenser head), UNIPLATE Collection and Analysis Microplate, 24-well, 10 ml, natural polypropylene, round well bottom, UNIPLATE Collection and Analysis Microplate, 96-well, 2 ml, natural polypropylene, round well bottom, UNIPLATE Collection and Analysis Microplate, 48-well, 5 ml, natural polypropylene, rectangular well, flat bottom, Cassette for fraction collection in deep-well plate, Cassette for fraction collection in 8 mL tubes, Rack for fraction collection in 250 mL bottles, Superloop, 1/16" fittings (KTAdesign), 50 ml, Tubing connector, 5/16" + Ferrule (yellow), 1/8", L9-1.2 and V9-C/V9-Cs/the connected column, Sample tubing kit for 7 inlets, i.d. Use the buffering ranges from Table 1 to select the eluent pH in which the analyte should be 100% ionised. Leakage on the wet side of the instrument. Check that the deep well plates are supported. Make sure that approved deep well plates are used. Clean the check valve, See Section 7.6.7 Clean the pump head check valves, on page 343 in the system handbook. A complete overhaul, once a year, ensures that your instrument is running at peak performance so you can be confident of your scientific results. The air intake on the rear or on the right side of the instrument is covered. Partially blocked solvent filters You will also find useful information in your user manuals. The current is cut off by a temperature sensitive component. The Superloop is filled to the max. The following recommendations go into more detail: Never leave the Multichannel Gradient Valve (MCGV) in water, buffer, or ACN fora long time. Contact ASI for the more information. (9) Acetonitrile is very soluble in water and is quite flammable. Do a search on this forum, there were different/updated valves which worked a lot better. 0.75 mm, Tubing Kit for Outlet Fractionation (10 outlets), System Tubing kit for KTA pure 150, i.d. Check calibration with a solution with known conductivity. All rights reserved. See KTA pure System Handbook, chapter Prepare the system for a run. See Section 4.3 Install inlet valves, on page 138 in the system handbook. Arc HPLC System Accurate analysis for all Ever-evolving regulations are impacting LC method approaches for product quality control and beyond. See Section 7.3.2 Action: Replace the check valve. The magnet should vibrate. Location and illustration of UV monitor U9-M, UV monitor U9-T, and UV monitor U9-L. If the retention time is shifting or jumping, oftentimes the MCGV is blocked (one or more channels do not open or close as planned and thus the composition is not correct). The pressure has increased due to increased viscosity. Action: If possible, place the Cassettes close to waste, i.e., position 5 or 6 during fractionation. We can help you design a schedule and routine to allow you to maintain your system, please contact your local Cytiva service representative. The individual instrument modules are presented in the table below. Tools Required: In System Control, select System:Settings. Increase the fraction size or lower the flow rate. See Section4.3 Install inlet valves, on page138 in the system handbook. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the KTA pure System Handbook. The reason could be that too many fraction collector instructions have been sent. To find out more about service possibilities contact your local Cytiva service representative. Three different Mixer chambers are available for KTA pure 25. See Section 4.3 Install inlet valves, on page 138 in the system handbook. Incident: One inlet is blocked, If this error is recurrent, generate a System error report and contact Service. It is not possible to vary the wavelength, or turn on or off the U9-L monitor. Hold the hand above the mixer. Air bubbles are passing through the flow cell. The System pressure monitor R9 has higher resolution than the other pressure monitors, and is therefore recommended for troubleshooting purposes. Clean the Drop sync sensor diode windows. Check that the Mixer chamber is free from solids. This Information Applies To: Agilent G1311A/B/C, G7111A/B, Quaternary Pumps, and G1310A/B, G7110B Isocratic Pumps, G1312A/B/C, G7112B Binary Pumps Clean the Cassette type code of the Cassette. The monitor is labelled C9. We will concentrate on the simple ball-type valve here; for more information on the active check valve, see reference 1. Unfortunately, the SEM images were not included in the copy of the patent I was able to obtain, but the descriptions were sufficient for our purposes. Perform a Fraction collector wash, see Section 8.6.4 Clean Fraction collector F9-C in the KTA pure System Handbook. The capillary is used during the System performance tests. A pressure or leak rate test in Lab Advisor suggests the inlet valve may require replacement. Reset GPV and select OK. Prime unit at 7.5 ml for 3-5 minutes. Check that the detector is fitted correctly. See Deep well plates, section 3.2.3 Cassettes, Cassette tray and racks, in the KTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service. . Check the module's Node ID. However, note that a larger Mixer chamber distorts and delays the gradient. Learn how toeffectively troubleshoot your HPLC pump: We are sorry this was not helpful. Used seats that still worked had smooth patches on the surface, and failed seats had even smoother surfaces. FT-IR experiments identified components of the residue as aliphatic amines, esters, and possibly ethers. This Information Applies To: Agilent 1260 Isocratic (G1310B) quaternary (G1311B) pump modules See illustration below. Specifications. See KTA pure System Handbook, chapter Prepare the system for a run. The flowrate may need to be corrected. NOTE Filtering through nylon filters is not recommended for High Sensitivity LCMS. Use a temperature compensation factor. Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques. Issue: Precision of peak area and linearity are shown in figures 7 and 8. Full scan will not work with prefilled wells during these conditions. Purge pumps. He takes a look at them in this month's installment of "LC Troubleshooting.". The patent examined the reversible nature of the surface change by taking a seat that had been observed to have a smooth surface and exposing it to a 1400 F flame for 10 min. Check solution absorption and that the cell is fitted correctly. Store the column in an appropriate solvent (check column information sheet) I again thank all the readers who reported their experiences to me. Restart the instrument. You can purge all channels using a composition of 25 % for each channel, using warm water in all four channels. Steps to follow Some of the topics include: My pump has erratic flow rates My pump has no flow out the outlet check valve I'm noticing pressure fluctuation In the Manual Instructions dialog, decrease the I factor of the Pressure control parameters instruction. Check Node ID and change the Node ID according to Node ID, on page 465 in the system handbook. Do not use Mixer chamber 15 ml with an KTA pure 25 configuration. If one of the valves is the source of the blockage, and acetonitrile (ACN) is one the solvents used in the LC, this guide may help you recover the system. Maximum operating temperature is 35C. Replace the piston seal and rinse membrane according to Section 7.8.9 Replace pump piston seals of Pump P9, on page 381 in the system handbook. Check the connections on the back of the instrument. Check the tubing connectors. Correct the Detector - Outlet valve delay volume. This residue causes the surface to become so smooth that the ruby ball sticks to it as a result of surface tension, not chemical interactions. Check that the pump is operating properly. The Cassette is turned in the wrong direction. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the KTA pure System Handbook for information of how to place the deep well plate. Tighten the connector. See pH monitor and pH valve, on page409 in the system handbook. The waste tubing is mounted on delivery. The combination of high flow rates and liquids with low surface tension might lead to spillage in the Fraction collector. Clean the connectors. PED Directive 97/23/EC. ACN is a by-product from the polymer industry, and therefore has a strong tendency to self-polymerize. So what can you expect from a planned maintenance visit from Cytiva service representative? Check that the Mixer chamber is free from solids. The illustration below shows the location of System pump A and System pump B, together with a detailed view of a system pump. The air intake on the rear or on the left side of the instrument is covered. Remove Inlet valve A2 and change the Node ID. Filter ACN using a 0.45 m nylon filter depending on initial solvent quality (unless you use High Sensitivity LC/MS). Inlet tubing Wait for a while and try again. Remove the valve and make sure that the cable is connected Instruction, on page 203 in the system handbook. This article shows different options to troubleshoot low pressure or no flow issues in 1100/1200 series Isocratic/Quaternary/Binary pumps. This Information Applies To: Agilent Liquid Chromatography (LC) systems The best source of information I have found is a check-valve patent (4) brought to my attention by one of the readers (thank you, B.H.). Make sure that the cord is attached using the clip, thereby preventing the cord from coming loose. Select Mixer chamber OR Issues related to UV monitor and UV detector unit, Large difference in refractive index between buffer A and buffer B. Check the external equipment connected to the I/O-box. Purge the pumps. See Examples of pump pressure curves, on page 427 in the system handbook for examples of pump pressure curves. Remove the valve and make sure that the cable is connected. Clean the UV flow cell, see Section 7.5.1 Clean the UV flow cell, on page 318 in system handbook.
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